The diagnostic value of EB virus antibody combined with atypical lymphocyte detection in pediatric IM
EB病毒抗体联合异型淋巴细胞检测在儿童IM的诊断价值研究

摘要

目的 探讨异型淋巴细胞和EB病毒抗体联合检测对儿童IM的辅助诊断意义。方法 本研究选取本院于2022年6月到2024年3月间住院的传染性单核细胞增多症患儿53例作为IM组，另选取非传染性单核细胞增多症发热患者60例作为非IM组。对所有患儿分别进行异型淋巴细胞检测和EB病毒抗体（VCA-IgM）测定，并对其进行统计分析，评估各种检测手段单个或组合对儿童的诊断价值。结果 53例患儿异型淋巴细胞比例（16.22±3.47）%，异型淋巴细胞阳性率62.26%（33/53），VCA-IgM抗体的阳性检出率为83.02%（44/53）。其中，异型淋巴细胞比例测定与VCA-IgM检测的结合，其敏感性与准确性都较单独应用高。联合检测与单独异型淋巴细胞检测及单独VCA-IgM抗体测定的特异度、阳性、阴性预测值均有显著差异（P<0.05）。结论 异型淋巴细胞检测及VCA-IgM联合检测可作为小儿IM的辅助诊断指标。

Abstract

Objective To explore the auxiliary diagnostic significance of combined detection of atypical lymphocytes and EB virus antibodies for pediatric IM.
Methods This study selected 53 children with infectious mononucleosis who were hospitalized in our hospital between September 2023 and February 2024 as the IM group, and another 60 non infectious mononucleosis fever patients as the non IM group. All patients were tested for atypical lymphocytes and EB virus antibodies (VCA IgM), and statistical analysis was performed. Evaluate the diagnostic value of various detection methods, whether single or combined.
Results The proportion of atypical lymphocytes in 53 patients was (16.22 ± 3.47)%, and the positive rate of atypical lymphocytes was 62.26% (33/53). The positive detection rate of VCA IgM antibodies was 83.02% (44/53). Among them, the combination of heterotypic lymphocyte proportion determination and VCA IgM detection has higher sensitivity and accuracy compared to single application. There were significant differences (P<0.05) in the specificity, positive, and negative predictive values between joint testing, individual heterotypic lymphocyte testing, and individual VCA IgM antibody testing.
Conclusion   The detection of atypical lymphocytes and the combined detection of VCA IgM can serve as auxiliary diagnostic indicators for pediatric IM.